Sedimentation Velocity Analytical Ultracentrifugation
نویسنده
چکیده
(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a single solute. For an interacting system the distribution is an absolute reflection of the mass action relationship between the species participating in the chemical equilibrium reaction (concentrations as well as molecular masses of the participating species).
منابع مشابه
Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation
Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Title Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Publication Type Journal Article Year of Publication 2005 Authors Balbo, A, Minor, KH, Velikovsky, CA, Mariuzza, RA, Peterson, CB, Schuck, P Journal Proc Natl Acad Sci U S A Volume 102 Is...
متن کاملCharacterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation.
This unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of self-association, heterogeneous association, multi-protein complexes, binding stoichiometry, and the determination of association constants. The analytical tools described include sedimentation coefficie...
متن کاملA new adaptive grid-size algorithm for the simulation of sedimentation velocity profiles in analytical ultracentrifugation
Analytical ultracentrifugation allows one to measure in real-time the concentration gradients arising from the application of a centrifugal force to macromolecular mixtures in solution. In the last decade, the ability to efficiently solve the partial differential equation governing the ultracentrifugal sedimentation and diffusion process, the Lamm equation, has spawned significant progress in t...
متن کاملCharacterization of heterologous protein-protein interactions using analytical ultracentrifugation.
Methods for quantitative characterization of heterologous protein-protein interactions by means of analytical ultracentrifugation (AUC) include sedimentation equilibrium, tracer sedimentation equilibrium, sedimentation velocity, and analytical band sedimentation. Fundamental principles governing the behavior of macromolecules in a centrifugal field are summarized, and the application of these p...
متن کاملSedimentation velocity spins a new weave for an old fabric.
Sedimentation velocity analysis is a powerful tool for the investigation of biological macromolecules under a wide range of solution conditions. If carefully applied, it can be an effective tool for the characterization of interacting systems in solution. It is rapidly becoming a method of choice among the biotechnology community. In recent years, there have been notable advances in the ease of...
متن کاملMacromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.
Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particles, determined from the experimental data, which scales the diffusion coefficie...
متن کامل